Analysis Note

Positive ControlRat naphe nuclei, hypothalamus, cortex, or spinal cord

Application

Frozen Sections (1:10,000-1:15,000; see application references) Immunoblotting (see comments)Immunofluorescence (1:200-1:400)

General description

Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the serotonin (5-HT) transporter protein.

This Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb is validated for use in Frozen Sections, Immunofluorescence for the detection of Serotonin (5-HT) Transporter (602-622).

Recognizes the serotonin (5-HT) transporter in hypothalamus raphe nuclei and spinal cord.

Immunogen

a synthetic peptide corresponding to amino acids 602-622 of rat 5-HT transporter

Rat

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Zhou, F.C., et al. 1996. Mol. Brain Res.43, 267.Blakely, R.D., et al. 1994. J. Exp. Biol.196, 263.Coccaro, E.F. and Murphy, D.L. (Eds.) 1990. in Serotonin in Major Disorders. The Progress in Psychiatry Series, American Psychiatric Press, Washington, D.C.

Antibody specificity was examined in the rat cortex, hypothalamus, raphe nuclei, and spinal cord. Staining is completely eliminated by pretreatment of antibody with Serotonin (5-HT) Transporter Control Peptide (Cat. No. PP87) at a concentration of 5 µg/ml. Low levels of glutaraldehyde (0.1%-0.3%) may be used in conjunction with paraformaldehyde for tissue perfusion fixation. The specificity of this antibody was also determined by immunoblotting analysis using rat brain extracts of the cortex, hypothalamus, midbrain, and hindbrain, which specifically detected a single band. Antibody should be titrated for optimal results in individual systems.

Packaging

100 µL in Plastic ampoule

Physical form

Undiluted serum.

Reconstitution

Reconstitute the lyophilized antibody with 100 µl of sterile distilled H₂O. Reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Further dilute with sterile PBS or Tris buffer to dilutions no higher than 1:10.

Warning

Toxicity: Standard Handling (A)